Artificial Cerebrospinal Fluid
| Chemical |
M.W. |
mM |
g/litre |
| NaHCO3 |
84.0 |
26 |
2.18 |
| NaCl |
58.45 |
124 |
7.25 |
| CaCl2 |
110.99 |
2 |
0.22 |
| Glucose |
180.16 |
10 |
1.80 |
| MgSO4 |
120.38 |
2 |
0.24 |
| KCl |
74.55 |
5 |
0.37 |
| KH2PO4 |
136.09 |
1.25 |
0.17 |
Instructions:
Gradually add all the ingredients except CaCl2 to 900 ml of distilled H2O while stirring. Bring up the volume to 1 litre. Only add the CaCl2 when the solution is being oxygenated or it will not go into solution.