Gelatin - Chrom Alum Coated Microscope Slides

Ingedients:

• 800 ml Distilled H₂O
• 4.0 g Gelatin
• 400 mg Chromium Potassium Sulfate

Procedure :

1. Measure out the water and  pour it into a beaker. Place the beaker on a hot plate at low heat with a stir bar at medium speed.
2. Slowly add the gelatin allowing it to dissolve.
3. Once the gelatin has dissolved, add the chromium potassium sulfate.
4. Optional step: filter the gelatin / chrom alum solution.
5. Pour the solution into the large staining dish that you will use for dipping the slides and allow the solution to cool to room temperature.
6. Place the microscope slides into the large staining racks.  (You should start with clean, dry slides. Depending on the supplier, you may have to clean the slides prior to coating).
7. Place each rack of slides to be coated into the gelatin solution for about 1 minute. Agitate the rack gently to shake off any air bubbles on the slides. Remove the rack slowly from the solution.
8. Allow the slides to dry in the racks overnight in a 40-50^o C oven.
9. Remove the slides from the racks and return them to their boxes. Clearly label the boxes.